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Mostreports on the use of molecular tools to detect bacteria in PJI have been based onamplification of a broad-range bacterial target, such as the 16S ribosomal RNA (rRNA)gene, with subsequent identification of the organism detected by sequencing of amplifiedDNA.

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However, recurrence ofheadache within 24 hr has been noted in20–40% patients, probably due to short t? ofsumatriptan.

Instead of collecting the bone and blood samples at the same time point afterthe dose for all patients, samples should be spread out over a time period to support PKmodeling. Many of these assays were initially developed byor for the pharmaceutical industry for the testing of thou-sands to millions of compounds against molecular targetsto find lead compounds against specific diseases (Bleicheret al., 2003; Mayr and Bojanic, 2009).

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